BBa_K284007

BBa_K284007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K284007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Maria Carolina de Barros Grassi

Date created: 2009-08-05 11:00:00

Date modified: 2015-05-08 01:11:48

tra operon promoter (Py)

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K284007_sequence (Version 1)

Description

Py is the tra operon promoter of F-plasmid. It is repressed by the host nucleoid-associated protein, H-NS and activeted by TraJ protein. Conflicting evidence suggests that TraY can act as both an activator and a repressor.
The tra operon encodes F-plasmid proteins (except TraJ and TraM) involved in DNA transfer by conjugation in E.coli.

Notes

The mechanism for controlling transcription from Py remains undefined.
The Py region contains significant intrinsic curvature, which could interfere in the regulation.

Source

F-plasmid of Escherichia coli.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Tray binding site	50,75	feature/binding, non_covalent_binding_site
-35	11,16	feature/promoter, promoter
-10	34,39	promoter, feature/promoter

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K284007/1

Member of these Collections

• iGEM Parts Registry