BBa_K284008

BBa_K284008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K284008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fabiana de Melo Duarte, Leonardo Minete, Maria Carolina Grassi and Lucas P. Parizzi
Date created: 2009-08-05 11:00:00
Date modified: 2015-05-08 01:11:48

tra operon promoter (Py)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K284008_sequence (Version 1)

Description

Py is the tra operon promoter of F-plasmid. It is repressed by the host nucleoid-associated protein, H-NS and activeted by TraJ protein. Conflicting evidence suggests that TraY can act as both an activator and a repressor. The tra operon encodes F-plasmid proteins (except TraJ and TraM) involved in DNA transfer by conjugation in E.coli. The regions shows intrinsic curvature, which could interfere in regulation. For this reason, the two closer bends upstream promoter region are included.

Notes

The mechanism for controlling transcription from Py remains undefined. The Py region contains significant intrinsic curvature, which could interfere in regulation

Source

F-plasmid of Escherichia coli.

Sequence Annotation Location Component / Role(s)
Tray binding site
-35
-10
bend
bend
108,133
69,74
92,97
47,68
17,44
feature/binding non_covalent_binding_site
feature/promoter promoter
promoter feature/promoter
sequence_feature feature/misc
feature/misc sequence_feature
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K284008/1