Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Charoy
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:49
LacI repressor and a double terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K292005_sequence (Version 1) |
Description
This part contains a LacI repressor and a terminator (BBa_C0012 + BBa_B0014). The LacI repressor repressed pLac promoter from the E.coli lactose operon. So LacI repressor is really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI and creates a failed feedback mechanism for pLac inhibition.History:
In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
Notes
When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A2.Source
Part:BBa_C0012, Designed by Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton Group: RegistryPart:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16).