BBa_K299105

BBa_K299105 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K299105
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Olchowik
Date created: 2010-08-06 11:00:00
Date modified: 2015-05-08 01:11:50

J23100xbaJ61100



Types
DnaRegion

Roles
engineered_region

Intermediate

Sequences BBa_K299105_sequence (Version 1)

Description

J61100 is an RBS and comes in a in plasmid pSB1A2, but there is also a J23100 promoter and XbaI site in the vector before J61100 RBS.

Biobrick 5' XbaI J23100 XbaI RBS Part Biobrick 3'
gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagaGAAAGAGGGGACAAactagtagcggccgctgcag

"Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely "T". The "G" downstream of the XbaI site obeys the standard. Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position."

The sequence of 61100 has been copied here together with the promoter sequence and the xba site to enable accurate in silico assembly of composite parts.

Source

Distribution.

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K299105/1