BBa_K299201

BBa_K299201 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K299201
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Olchowik
Date created: 2010-08-08 11:00:00
Date modified: 2015-05-08 01:11:50

J23100[promoter].K299107[TxbaIG].J61101[RBS] **no b-brick scars between**



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K299201_sequence (Version 1)

Description

J23100[promoter].K299107[xbaIG].J61101[RBS] =
Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.

Also be careful part J61101 is in registry as AAAGACAGGACCC, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:

AAAGACAGGACCC**a** (and then comes rest of the suffix: ctagt)

It means thatThe physical sequence is different than the record in registry for J61101, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway.

This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector
with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.

The original Anderson RBSes and vector are described here.

http://partsregistry.org/wiki/index.php?title=Part:BBa_J61101

Source

Registry. It is J61101 with part of its original vector.

http://partsregistry.org/wiki/index.php?title=Part:BBa_J61101

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K299201/1