Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Olchowik
Date created: 2010-08-08 11:00:00
Date modified: 2015-05-08 01:11:50
J23100[promoter].K299107[TxbaIG].J61100[RBS] No b-brick scars between
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K299300_sequence (Version 1) |
Description
J23100[promoter].K299107[xbaIG].J61101[RBS] =Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.
Also be careful part J61100 is in registry as AAAGAGGGGACAA, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:
AAAGAGGGGACAA**a** (and then comes the rest of the suffix: ctagt)
It means that the physical sequence is different to the record in registry for J61100, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway.
This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector
with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.
The original Anderson RBSes and vector are described here.
http://partsregistry.org/wiki/index.php?title=Part:BBa_J61100
Source
Registry. It is J61100 with part of its original vector.http://partsregistry.org/wiki/index.php?title=Part:BBa_J61100
| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_K299107 BBa_J61100 | BBa_J23100 BBa_K299107 BBa_J61100 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K299107 BBa_J61100 | 1,35 36,43 44,55 | BBa_J23100 BBa_K299107 BBa_J61100 |