Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Olchowik
Date created: 2010-08-08 11:00:00
Date modified: 2015-05-08 01:11:50
J23100[promoter].K299107[TxbaIG].J61127[RBS] No b-brick scars between
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K299327_sequence (Version 1) |
Description
J23100[promoter].K299107[xbaIG].J61127[RBS] =Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.
In this case the physical DNA mathes the registry sequence. After assembly part J61117 is in registry as AAAGAGTGGAAC, The functional DNA of this RBS (part J61127) is AAAGAGTGGAAC*T*, when suffix is added, the first T of the suffix gives 'AAAGAGTGGAACT' sequence.
This part is just to enable automatic in sillico assembly of the Anderson's RBS collection in the original vector with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.
The original Anderson RBSes and vector are described here.
http://partsregistry.org/wiki/index.php?title=Part:BBa_J61127
Source
Registry. It is J61117 with part of its original vector.http://partsregistry.org/wiki/index.php?title=Part:BBa_J61127
| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_K299107 BBa_J61127 | BBa_J23100 BBa_K299107 BBa_J61127 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K299107 BBa_J61127 | 1,35 36,43 44,55 | BBa_J23100 BBa_K299107 BBa_J61127 |