Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michał Lower
Date created: 2010-10-15 11:00:00
Date modified: 2015-05-08 01:11:50
minC under pT7 and B0034
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K299808_sequence (Version 1) |
Description
Construct for testing minC cell division inhibitor protein. This construct was generating no transformants in BL21 strain in no induction conditions. This was probably due to leaky expression of MinC from pT7 that resulted in constitutive growth inhibition. However we were able to work with it in E. coli Top10 to confirm it's correctness.Source
ligation of K299806 and K299509| Access | Instance | Definition |
| public public public | BBa_I719005 BBa_B0034 BBa_K299806 | pT7 BBa_B0034 BBa_K299806 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I719005 BBa_B0034 BBa_K299806 | 1,23 32,43 50,745 | pT7 BBa_B0034 BBa_K299806 |