BBa_K300006

BBa_K300006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K300006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Susanna Zucca, Federica Sampietro, Paolo Magni

Date created: 2010-10-03 11:00:00

Date modified: 2015-05-08 01:11:51

mOrange with ADH1 transcriptional terminator for yeast

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K300006_sequence (Version 1)

Description

This part can be assembled with a yeast promoter(+RBS) upstream to drive mOrange (BBa_E2050) expression.

The BBa_K165018 part (NLS-Term with RFC23 Prefix and Suffix) has been used in this BioBrick in order to yield the transcriptional termination, while the nuclear localization signal does not work (it is after the stop codon of mOrange and the BioBrick scar TACTAGA forms another stop codon and send the translation out of frame).

Notes

We used BioBrick Standard Assembly.

Source

BBa_E2050 - from 2010 DNA Distribution;
BBa_K165018 - from 2010 DNA Distribution.

Components

Access	Instance	Definition
public	BBa_E2050	mRFP1
public	BBa_K165018	NLS+Term

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_E2050	1,769	mRFP1
BBa_K165018	778,1030	NLS+Term

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K300006/1

Member of these Collections

• iGEM Parts Registry