BBa_K300984

BBa_K300984 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K300984

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Manuel Lupotto, Lorenzo Pasotti, Paolo Magni

Date created: 2010-10-08 11:00:00

Date modified: 2015-05-08 01:11:52

E. coli strain BW25141 (pir+)

Details

• Types: DnaRegion
• Roles: chromosome; Cell

Description

This E. coli strain can be used to propagate plasmids with R6K conditional replication origin (BBa_J61001, BBa_K300998), such as BBa_K300000 integrative base vector after the excision of its insert (BBa_I52002).

BW23474 propagates R6K origin at a copy number comparable to medium copy plasmids (~15 plasmids per cell) [1].

Genotype:

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514

Notes

This is not the only strain able to propagate R6K at medium copy, but it has been mentioned in several papers and it is easily retrievable from the CGSC.

Source

Coli Genetic Stock Center: strain number CGSC#7635.

Other Properties

• igem#experience: Works
• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K300984/1

Member of these Collections

• iGEM Parts Registry