BBa_K302012

BBa_K302012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K302012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rachel May Boyd, Phil Hall
Date created: 2010-08-11 11:00:00
Date modified: 2015-05-08 01:11:52

IPTG-inducible filamentous cell formation



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K302012_sequence (Version 1)

Description

Bacillus subtilis in response to stress such as DNA damage stops the cells from dividing. This is a part of the SOS response initiated by the accumulation of single stranded DNA from DNA damage or stalled replication. Two proteins are vital for this response: RecA and LexA. RecA forms filaments on ssDNA and promotes the autocleavage of LexA. LexA usually represses the SOS operon. dinR is homologous to lexA in E. coli and is transcribed in the opposite direction of yneA.
yneA stops the formation of ftsZ ring indirectly. When ftsZ forms a 30 subunit ring at the midpoint of the cell, it will contract and cause cell division. By expressing yneA and inhibiting ftsZ ring formation, the cells will grow filamentous.
By inhibiting cell division, yneA allows the DNA damage genes to repair the DNA damage before continuing with the cell division cycle. It is hypothesized that yneA acts through an unknown transmembrane protein to inhibit ftsZ ring formation; we call this/these unknown components ???Blackbox proteins???. As the evidence shows expression of yneA leads to filamentation.

Notes

none

Source

Genomic DNA from Bacillus subtilis -yneA
Hyperspankoid from oid and hyperspank

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BBa_K302012/1