Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Thai Phi
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:11:53
pMec-7::eCFP::unc-54 3' UTR (for use in C. elegans chassis)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K309032_sequence (Version 1) |
Description
This is a construct consisting of a Mec-7 promoter, eCFP, and an unc-54 3'UTR. Worms transformed with this construct will emit light at 477 nm from the touch receptor neurons when excited with light at 434 nm.Notes
Parts PCRed out of genomic/plasmid DNA and ligated together using T4 ligase. A Kozak sequence was added in front of the reporter (eCFP) in order to facilitate the translation process.Source
From C. elegans (pMec-7, unc-54 'UTR)and A. victoria (eCFP) DNA.| Access | Instance | Definition |
| public public public | BBa_K309000 BBa_K309020 BBa_K309012 | BBa_K309000 BBa_K309020 BBa_K309012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K309000 BBa_K309020 BBa_K309012 | 1,721 730,1458 1467,2358 | BBa_K309000 BBa_K309020 BBa_K309012 |