BBa_K310000

BBa_K310000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K310000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Francis Lee
Date created: 2010-09-14 11:00:00
Date modified: 2015-05-08 01:11:53

micA (sRNA)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K310000_sequence (Version 1)

Description

micA is a small non-coding RNA regulator that binds to the 5' UTR of OmpA via non-perfect base pairing. The primary action of micA is the translational repression of OmpA by ribosome occlusion of OmpA mRNA and subsequent destabalization of the transcript. micA binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex.

Notes

The biobrick prefix is attached just upstream of the sRNA. This leads to the addition of 8 nucleotides (scar coding) to the 5' end of the sRNA, TACTAGAG, when combined with an upstream part (ie-promoter). All sRNA's in E. coli carry an intrinsic terminator. The suffix is located ~20 bp downstream of this terminator.

Source

Escherichia coli MG1655

igem#experience Works  
igem#sampleStatus Not in stock  
igem#status Unavailable  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K310000/1