Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin Borchardt
Date created: 2010-09-23 11:00:00
Date modified: 2015-05-08 01:11:53
ArsR under the control of J23100, includes RBS B0034 and terminator B0015
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K310006_sequence (Version 1) |
Description
ArsR is a transcriptional repressor regulating the Ars Operon in E. coli. This biobrick includes ArsR under the control of the promoter J23100 and includes the RBS B0034 and terminator B0015Notes
I needed to perform site directed mutagenesis in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.Source
I used PCR to isolate ArsR from E. coli K-12 MG1655 genome and attached B0015, B0034 and J23100 from the parts registry.| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_B0034 BBa_K310007 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 BBa_K310007 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K310007 BBa_B0010 BBa_B0012 | 1,35 44,55 62,415 424,503 512,552 | BBa_J23100 BBa_B0034 BBa_K310007 BBa_B0010 BBa_B0012 |