BBa_K310008

BBa_K310008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K310008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin Borchardt

Date created: 2010-09-29 11:00:00

Date modified: 2015-05-08 01:11:53

B0034+ ArsR + B0015

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K310008_sequence (Version 1)

Description

ArsR, a transcriptional repressor of the Ars Operon hooked up with the RBS B0034 and the terminator B0015 for expression when placed under the control of a promoter.

Notes

Site directed mutagenesis was performed in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT

Source

E. coli MG1655 genome

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_K310007	BBa_K310007
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	1,12	BBa_B0034
BBa_K310007	19,372	BBa_K310007
BBa_B0010	381,460	BBa_B0010
BBa_B0012	469,509	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K310008/1

Member of these Collections

• iGEM Parts Registry