Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ethan Johnson, Claudia Schmidt-Dannert, Poonam Srivastava, Ian Windsor
Date created: 2010-10-14 11:00:00
Date modified: 2015-05-08 01:11:54
Constitutive lac promoter with downstream EGFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K311002_sequence (Version 1) |
Description
The mutations were generated to get a constitutive lac promoter. The promoter was cloned in pSB1C3 upstream to the EGFP. The promoter activity was checked by transforming the recombinant plasmid in Lac repressor negative (TOP10) and Lac repressor positive (DH5 alpha pro) E. coli cells. The recombinant cells were grown in the presence of varying concentration of inducer (IPTG) and also in the absence of the inducer. The culture was collected at different time points post induction and promoter activity was measured by measuring the fluorescence intensity under FACs. Both the cell types show constitutive behavior of the promoter.Notes
The lac and EGFP were obtained from one of our home made plasmids by digesting it with EcoR I and Pst I. The part was ligated to EcoR I and Pst I cut pSB1C3.Source
genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| RBS-3\Weak BBa_B0032 RBS GFP protein BBa_E0040 BBa_B0016 | 1,13 1,13 7,10 20,739 20,739 748,795 | feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/rbs ribosome_entry_site CDS feature/protein feature/BioBrick engineered_region feature/BioBrick engineered_region |