Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Adams, Swati Choudhary, Anthony Goering, Rachel Farr, Ethan Johnson, Annie Kathuria, Poonam Srivastava, Ian Windsor
Date created: 2010-10-14 11:00:00
Date modified: 2015-05-08 01:11:54
Complement of Ethanolamine Utilization Microcompartment proteins from Salmonella LT2
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K311004_sequence (Version 1) |
Description
Three proteins subunits of a bacterial micro-compartment. The genes are in the pSB1C3 plasmid. A total of 5 micro-compartment genes are cloned in a single plasmid. They are arranged as two sets of two genes (in a single operon) with one gene which is upstream to the pairs. They are flanked directly upstream by a mutated Lac promoter to make them constitutively expressed. The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes.Notes
The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes. The genes were ligated into a modified pUC biobrick vector containing a constitutive promoter. The genes were taking in their native operons and ligated sequential according to size.Source
Salmonella enterica LT2 genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| modified plac Eut S modified plac EutM EutN modified plac EutL EutK BBa_K311004 | 1,124 140,475 556,679 695,985 1086,1385 1466,1589 1605,2264 2277,2771 1,2771 | feature/promoter promoter feature/misc sequence_feature feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter sequence_feature feature/misc sequence_feature feature/misc feature/BioBrick engineered_region |