| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K315021_sequence (Version 1)
|
Description
This sequence is the wild type TetA gene which has been deoptimized from base pairs 144 to 290. This is a basic part that can be placed behind a promoter and produce the Tetracycline resistance protein. Being that this gene is deoptimized, we are hypothesizing that it will allow for less translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis)
Notes
During the design of this sequence, it was necessary to ensure that the deoptimized sequence did not contain BioBrick restriction sites and that the translational sequence was identical to that of the wild type gene
Source
This TetA gene has been deoptimized from base pairs 144 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was constructed using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.
