Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nitya Rao
Date created: 2010-07-20 11:00:00
Date modified: 2015-05-08 01:11:56
pBad + RBS + cre + pTet + loxP forward + RBS + RFP + lox5171 forward
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K315032_sequence (Version 1) |
Description
This part consists of cre recombinase behind a weak promoter and a floxed RFP gene after a stronger promoter. This construct can be used to determine how various lox sites recombine and whether cre has different affinities for different lox sites.Notes
Cre recombinase is behind a weaker promoter to prevent the protein from excising all of the RFP genes in the cell. However, RFP is behind a stronger promoter because a minimal level of protein production is needed for it to be a useful reporter.Source
This part was created in our lab.| Access | Instance | Definition |
| public public | BBa_I718008 BBa_K315030 | BBa_I718008 BBa_K315030 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I718008 BBa_K315030 | 1,2276 2285,3154 | BBa_I718008 BBa_K315030 |