BBa_K315034

BBa_K315034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K315034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nitya Rao
Date created: 2010-07-20 11:00:00
Date modified: 2015-05-08 01:11:56

pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + lox5171 reverse



Types
DnaRegion

Roles
Measurement

engineered_region

Sequences BBa_K315034_sequence (Version 1)

Description

This part consists of cre recombinase behind a weak promoter and a floxed RFP gene after a stronger promoter. This construct can be used to determine whether inversions occur when variant lox sites are exposed to cre recombinase.

Notes

Cre recombinase is behind a weaker promoter to prevent the protein from excising all of the RFP genes in the cell. However, RFP is behind a stronger promoter because a minimal level of protein production is needed for it to be a useful reporter.

Source

This part was created in our lab.

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K315034/1