Pveg

BBa_K316001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K316001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-11 11:00:00
Date modified: 2015-05-08 01:11:56

pVeg Constitutive promoter for Veg locus from B. subtilis



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K316001_sequence (Version 1)

    Description

    This part is identical to the sequence submitted by Imperial 2008 team, this part was produced from K143053 by PCR.
    PVeg is a constitutive promoter controlled by Sigma factor A. This promoter has two binding sites which leads to high expression of downstream genes.
    There is some evidence that the sporulation master regulator the spoOA can interact with pVeg although the mechanism is not known.

    Notes

    PCR using Pfu polymerase to avoid mutations

    Source

    PCR from existing biobrick K143053 using Pfu polymerase II

    Sequence Annotation Location Component / Role(s)
    Sigma A-35
    Sigma A-35
    		63,68
    86,91
    		feature/promoter promoter
    promoter feature/promoter

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K316001/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)