Pveg

BBa_K316001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-11 11:00:00

Date modified: 2015-05-08 01:11:56

pVeg Constitutive promoter for Veg locus from B. subtilis

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K316001_sequence (Version 1)

Description

This part is identical to the sequence submitted by Imperial 2008 team, this part was produced from K143053 by PCR.
PVeg is a constitutive promoter controlled by Sigma factor A. This promoter has two binding sites which leads to high expression of downstream genes.
There is some evidence that the sporulation master regulator the spoOA can interact with pVeg although the mechanism is not known.

Notes

PCR using Pfu polymerase to avoid mutations

Source

PCR from existing biobrick K143053 using Pfu polymerase II

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Sigma A-35	63,68	feature/promoter, promoter
Sigma A-35	86,91	promoter, feature/promoter

Other Properties

• igem#experience: Works
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316001/1

Member of these Collections

• iGEM Parts Registry