Types | DnaRegion
|
Roles | DNA
sequence_feature
|
Sequences | BBa_K316002_sequence (Version 1)
|
Description
Dif sites are naturally found in B.subtilis and are used by this organism during genome replication.
Notes
The dif site was made by oligos designed to make overhangs for EcoRI and SpeI ( and ) or XbaI and PstI ( and ) to be used in standard Biobrick or 3A cloning.
Source
The dif sites were made by annealing synthestised oligoes.