BBa_K316008

BBa_K316008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-20 11:00:00

Date modified: 2015-05-08 01:11:56

Cleavable GFP-XylE fusion with Pveg promoter and terminator

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K316008_sequence (Version 1)

Description

This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. Terminator BBa_B0014 has been added to comply with Biobrick standards. This particular terminator is stronger and is different from BBa_B0015.

Notes

Standard Biobrick assembly

Source

Existing Biobricks BBa_K143053, BBa_K316005, BBa_B0015

Components

Access	Instance	Definition
public	BBa_K143053	Pveg-spoVG
public	BBa_K316006	GFP-XylE
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143053	1,117	Pveg-spoVG
BBa_K316006	124,1845	GFP-XylE
BBa_B0014	1854,1948	BBa_B0014

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316008/1

Member of these Collections

• iGEM Parts Registry