BBa_K316009

BBa_K316009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-24 11:00:00

Date modified: 2015-05-08 01:11:56

LacI inducible Fast Response Module, using cleavable XylE

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K316009_sequence (Version 1)

Description

5' his tagged GFP-TEV linker-XylE construct is pre-made in the cell under constitutive promoter. Both Pveg promoter and spoVG RBS are best suited for maximal expression in B.subtilis. While GFP is attached to the XylE monomer via the TEV cleavable linker, the catalytic activity is low. Transcription of TEV protease allows cleavage of the linker between GFP and XylE, thus XylE is free to tetramerise into a fully functional enzyme. XylE is then able to act as described in BBa_k316004

Please see 'Part Design' section for design considerations and parts used.

Notes

Standard biobrick assembly http://partsregistry.org/Assembly:Standard_assembly

Source

Made from parts - BBa_k316012 and BBa_k316008

Components

Access	Instance	Definition
public	BBa_K316017	BBa_K316017
public	BBa_K143053	Pveg-spoVG
public	BBa_K316006	GFP-XylE
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K316017	1,874	BBa_K316017
BBa_K143053	883,999	Pveg-spoVG
BBa_K316006	1006,2727	GFP-XylE
BBa_B0014	2736,2830	BBa_B0014

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316009/1

Member of these Collections

• iGEM Parts Registry