Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-25 11:00:00
Date modified: 2015-05-08 01:11:56
Reverse strand coding XylE
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K316011_sequence (Version 1) |
Description
This part contains XylE coding on the 3' strand. In many biological settings there may be read-through caused by upstream elements, however this can often be unidirectional. In settings where readthrough from 5' direction is expected to be much stronger than 3' direction, it may is advantageous to use 3' coding sequence instead of traditional 5'.For the purposes of characterisation, the part was made on the minus strand to reduce background transcription. Read-through from 5' direction may provide an additional benefit. Low quantities of anti-sence RNA may be produced as the result of 5' readthrough, which may in turn reduce basal expression sometimes seen in LacI systems.
Notes
Part was designed using several rounds of PCR, using Pfu polymerase to avoid mutations. For more information, please check our wikihttp://2010.igem.org/Team:Imperial_College_London/Strategy.Source
This part is a modified existing biobrick| Sequence Annotation | Location | Component / Role(s) |
| Start Codon Stop Codon XylE Gene | 922,924 1,3 4,924 | feature/start start_codon stop_codon feature/stop feature/cds CDS |