BBa_K316024

BBa_K316024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K316024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-22 11:00:00
Date modified: 2015-05-08 01:11:56

Chloraphenicol resistance gene with dif and 5amyE



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K316024_sequence (Version 1)

    Description

    This part is identical to BBa_K143023 except for the dif siteBBa_K3160 integrated behind the 5' amye BBa_K143001 integration sequence.

    Chloramphenicol acetyltransferase (CAT) BBa_J31005 confers resistance to chloramphenicol, a common lab selection antibiotic. The 5' amyE BBa_K143001 integration sequence allows integration into B. subtilis genome, which disrupts ability to breakdown starch. In order for integration to occur, 3' amyE integration sequence BBa_K143002 is also required.

    This part can be used as the 5' start of an amyE integration vector, the genes of interest can then be attached to the 3' end, followed by the 3' amye integratio sequwnce BBa_K143002.

    Notes

    Reverse PCR' was used to amplify the whole vector, while adding the dif sites by primer extension. Pfu polymerase was used to ensure error-free replication.

    Source

    Biobrick parts BBa_K143070 (which contains BBa_K143001, BBa_K143012, BBa_K143021) and BBa_K143064 (which contains BBa_K143031, BBa_B0015)

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K316024/1
     

    Attachments

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