Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yoshiyuki MURAKAMI
Date created: 2010-10-18 11:00:00
Date modified: 2015-05-08 01:11:57
SRII (NpSRII)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K317000_sequence (Version 1) |
Description
Halobacteria exhibit phototaxis responses to changes in light intensity and color using the seven-transmembrane retinylidene photoreceptors sensory rhodopsins I and II (SRI and SRII).Light-activated SRI and SRII transmit signals to their cognate transducers, HtrI and HtrII, respectively. The Htr proteins contain two transmembrane helices and cytoplasmic methyl-accepting and His-kinase-activating domains homologous to those of chemotaxis transducers of eubacteria,such as the Escherichia coli and Salmonella enterica serovarTyphimurium Tsr and Tar, chemotaxis transducers for serine and aspartate, respectively. Sensory rhodopsin
II from Natronobacterium pharaonis (NpSRII) is very similar in spectroscopic and functional properties to the repellent receptor SRII in Halobacterium salinarum, and it has been found to be more stable in response to variation in external conditions such as pH and ionic strength. The NpSRII protein mediates
a repellent response to blue-green light (maximum λ,497 nm) when it is coexpressed with its transducer, NpHtrII, in H. salinarum. Also, when expressed in E. coli, NpSRII is capable of binding all-trans retinal to form a blue-green-lightabsorbing pigment.