View

BBa_K318000

BBa_K318000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K318000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nathaniel Pantalone, Justin Vrana

Date created: 2010-08-26 11:00:00

Date modified: 2015-05-08 01:11:57

pBad + Cre + pLac + Hin + pCons + lacI

Back

VisBOL

Zoom

Truncate Labels

Details

Types	DnaRegion
Roles	engineered_region Generator
Sequences	BBa_K318000_sequence (Version 1)

Description

This composite part is an inducible recombinase generator. It can produce the Cre recombinase if induced with arabinose, and it can produce the Hin recombinase if induced with IPTG or an allo-lactose equivalent. It was refered to as the "Key Cassette" or "Key Construct" by the Wisconsin-Madison 2010 iGEM team.

Notes

Cells transformed with this part tend to grow slowly because of J23100.

Source

Registry

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_B1006 BBa_I718008 BBa_B1006 BBa_B1006 BBa_K200021 BBa_J31000 BBa_B1006 BBa_J23100 BBa_S03520 BBa_B1006	BBa_B1006 BBa_I718008 BBa_B1006 BBa_B1006 BBa_K200021 Hin BBa_B1006 BBa_J23100 BBa_S03520 BBa_B1006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B1006 BBa_I718008 BBa_B1006 BBa_B1006 BBa_K200021 BBa_J31000 BBa_B1006 BBa_J23100 BBa_S03520 BBa_B1006	1,39 48,2323 2332,2370 2379,2417 2426,2500 2507,3079 3088,3126 3135,3169 3178,4348 4357,4395	BBa_B1006 BBa_I718008 BBa_B1006 BBa_B1006 BBa_K200021 Hin BBa_B1006 BBa_J23100 BBa_S03520 BBa_B1006

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K318000/1