BBa_K318024

BBa_K318024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K318024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathaniel Pantalone, Justin Vrana
Date created: 2010-07-12 11:00:00
Date modified: 2015-05-08 01:11:57

pLac + RBS + Int (P22) + T



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K318024_sequence (Version 1)

Description

This composite part is an inducible P22 Int DNA integrase generator which acts on the attB and attP recombination sites. It is composed of the following biobricks: BBa_K200021, BBa_I11030, and BBa_B1006. A terminator was placed downstream of the Int encoding sequence to end transcription after the integrase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.

Notes

A terminator was placed downstream of the Int encoding sequence to end transcription after the integrase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.

Source

biobricks

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K318024/1