Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Perrudet Christian
Date created: 2010-10-23 11:00:00
Date modified: 2015-05-08 01:11:59
S + P25 + P28 + Ori + Tet
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K320014_sequence (Version 1) |
Description
Constitutive expression of P25 and P28 with an origin fro Asaia and E.Coli and a resiatance to Tetrafenicol in AsaiaThis BioBricks makes the bacteria resitance to Tetracycline, contain an origin of replication for Asaia/E.Coli and a constitutice expression of the protein P28.
The ookinete (plasmodium state in mosquito's gut) has been intensively studied by scientists, looking for an ideal transmission-blocking vaccine target. IGEM EPFL team's interest goes toward the ookinete surface membrane proteins P25 and P28. In fact, it has been shown that the binding of specific antibodies to these surface proteins was fatal to more than 99% of the parasites reference. Indeed, P25 and P28 seem to be essential to the ookinete survival, the crossing of the midgut epithelium and its transformation into an oocyst.
The team decided to design Asaia to express a soluble form of these 2 P-proteins. It could seems contra-intuitive to provide more of these proteins (by expression in Asaia) that are very important for the parasite survival, migration, and development. But our hypothesis is that adding a lot of these proteins in a soluble form will compete with the parasite surface protein and prevent the interaction necessary to its transmission. Because the functions of P25 and P28 are redundant, both proteins need to be inhibited or outcompeted to efficiently block the malaria transmission.
Notes
The coding for the P protein was AT to richa nd had to be optimized for Asaia and E.Coli.Source
This part has been designed from parts we made.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K320007 BBa_K320009 BBa_K320003 | 1,765 766,1533 1534,4223 | engineered_region feature/BioBrick engineered_region feature/BioBrick feature/BioBrick engineered_region |