| Types | DnaRegion
|
| Roles | engineered_region
Translational_Unit
|
| Sequences | BBa_K322007_sequence (Version 1)
|
Description
This is the lumazine protein of Photobacterium phosphoreum. Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein).
Notes
We submitted a version of lumP last year (BBa_K216007), but made a new one since we were having some issues working with our clone (lots of red colonies). So we made a new one in pSB1C3 starting from the original PCR product. On sequencing this (as well as looking at the sequence results from the original plasmids, M1117 and M1118) it was found that the sequence differed from the one we had submitted in a number of mutations, some silent, some not.
Source
Source: genomic DNA of Photobacterium phosphoreum.
