Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Rostain
Date created: 2010-08-31 11:00:00
Date modified: 2015-05-08 01:12:00
Photinus pyralis luciferase mutated for increased bioluminescence.
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K322451_sequence (Version 1) |
Description
Mutations Ile423Leu Asp436Gly and Leu530Arg increase the bioluminescence intensity by a factor of 12.5 according to Fujii et al. (2007). It is codon optimised for expression in E. coli.Reference
Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in
bioluminescence intensity of firefly luciferase using genetic modification. Analytical
Biochemistry 366, 131-136.
Notes
Wild type sequence with three mutations which increase relative bioluminescence.Reference
Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in
bioluminescence intensity of firefly luciferase using genetic modification. Analytical
Biochemistry 366, 131-136.
Source
DNA was synthesised to have the same amino acids as the wild type except for the three mutations.| Sequence Annotation | Location | Component / Role(s) |
| B0034 Brighter luciferase | 1,12 19,1674 | ribosome_entry_site feature/rbs feature/protein CDS |