BBa_K322624

BBa_K322624 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K322624

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Meng Lu

Date created: 2010-09-23 11:00:00

Date modified: 2015-05-08 01:12:00

Firefly luciferase S284T mutant

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain

Description

This is the same as the previous BioBrick but mutation S284T was introduced, which has been reported to result in a red-shifted output.

Features: S284T mutation 850 to 852

reference:

Notes

Sequence date: initial introduction of this mutation into K216015 (with PyeaR promoter and RBS) failed (sequence of 123 Aug 2010) and was repeated by Will and Lu. Positive results confirming the mutation were obtained by Lu for 9 of 14 clones on 7 Sep 2010. The mutated coding sequence was then amplified and cloned in pSB1C3. Sequence confirmation 22 Sep 2010; forward sequence is fine, reverse sequence good but missing the starting T of the suffix - goes straight from TAA to ACTAGT rather than TAATACTAGT. Compared to other mistakes in submitted BioBricks this is a pretty minor one, but still annoying.

Source

Photinus pyralis

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K322624/1

Member of these Collections

• iGEM Parts Registry