Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French and Maria Kowal
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:12:00
Marker construct which can be used to target tnaA in E.coli.
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K322710_sequence (Version 1) |
Description
This is a composite part containing the positive selection marker cat (chloramphenicol resistance) and the negative selection marker sacB (lethal to host in the presence of sucrose) fused to the up and down stream sequences of tnaA, the indole-producing gene. This construct can be used in the BRIDGE protocol for targeted deletion/replacement of tnaA in E.coli.Notes
We had to ensure there were no intrinsic restriction sites left between the sequences.Source
Fusion of parts from E.coli and B.subtilis as well as a designed part.| Access | Instance | Definition |
| public public public | BBa_K322705 BBa_K322922 BBa_K322706 | BBa_K322705 BBa_K322922 BBa_K322706 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K322705 BBa_K322922 BBa_K322706 | 1,478 487,3012 3021,3476 | BBa_K322705 BBa_K322922 BBa_K322706 |