Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French and Maria Kowal
Date created: 2010-09-23 11:00:00
Date modified: 2015-05-08 01:12:00
B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K322921_sequence (Version 1) |
Description
sacB encodes the Bacillus subtilis levansucrase,which is the enzyme catalyzing hydrolysis of sucrose and synthesis of levans(high-molecular-weight fructose polymers). It is lethal to gram-negative bacteria E-coli. It works with cat as an alternative method for inserting BioBricks into the genome by using homologous recombination instead of restriction digestion, with the added bonus of not leaving a marker behind in the product.Protocol is shown here: http://2010.igem.org/Team:Edinburgh/Project/Protocol
Notes
Note: MABEL was used to mutate an internal EcoRI site to GAGTTC (underlined).Features: coding sequence runs from 13 to 1434, RBS 1 to 3, EcoRI site mutated 211 to 216.
Source
Source: Bacillus subtilis 168 genomic DNA.Sequence (from SacBPlan11June10.docx)
| Sequence Annotation | Location | Component / Role(s) |
| RBS Coding sequence Removed EcoRI site | 1,3 13,1434 211,216 | ribosome_entry_site feature/rbs feature/protein CDS point_mutation feature/s_mutation |