Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maria Kowal and Chris French
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:12:00
Alternate targeting contruct for TnaA replacement using BRIDGE
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K322924_sequence (Version 1) |
Description
BRIDGE stands for BioBrick Recombineering In Direct Genomic Editing. It is an alternative method for inserting BioBricks into the genome by using homologous recombination instead of restriction digestion, with the added bonus of not leaving a marker behind in the product.The first step of BRIDGE requires the deletion of existing DNA (probably a non-coding piece or a non-essential gene) to introduce a construct of two genes; kanamycin resistance (BBa_K322923), and sacB (BBa-K322921), which prevents the host from growing on sucrose. After the first step we can select for cells which have taken up the construct by growing them on the relevant antibiotic.
Notes
This is an alternative to the cat-sacB system which uses chloramphenicol.Source
sacB is from B. subtilis| Access | Instance | Definition |
| public public public | BBa_K322705 BBa_K322923 BBa_K322706 | BBa_K322705 BBa_K322923 BBa_K322706 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K322705 BBa_K322923 BBa_K322706 | 1,478 487,2898 2907,3362 | BBa_K322705 BBa_K322923 BBa_K322706 |