Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julius Ho
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:02
AraC regulatory protein in RFC25 format
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K324001_sequence (Version 1) |
Description
This part consists of the AraC regulatory protein optimized with endings for protein fusion in the Freiburg assembly format (RFC25). There is also a 6-his tag appended to the C-terminus for easy purification using Ni-affinity column. The LVA rapid degradation tag from our source DNA, BBa_C0080, was also removed from the C-terminus. All modifications were made to BBa_C0080 by means of PCR with specially designed primer set.Notes
The LVA tag was removed because this part's intended purpose, harvesting from cells and purification, required the protein to have a long lifespan. 6-his tag was added to the C-terminus to avoid isolation of incompletely translated peptides during Ni-affinity purification.Source
refer to http://partsregistry.org/Part:BBa_C0080| Sequence Annotation | Location | Component / Role(s) |
| AraC in RFC25 | 1,924 | feature/protein CDS |