Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ben Reeve and Paul Masset
Date created: 2010-09-14 11:00:00
Date modified: 2015-05-08 01:12:02
EPIC Firefly Luciferase and LRE
P. Pyralis
(E. coli optimised)
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K325100_sequence (Version 1) |
Description
This is a mutant luciferase that is very much brighter than wild-type. It is supplied in an operon with an enzyme (LRE) that regenerates its substrate. When supplied with luciferin and placed under a promoter it will emit light at a green wavelength. If D-cysteine is added the LRE will be able to regenerate the luciferin substrate.Notes
It was ensured that no unwanted restriction sites were created during optimisation. A BamH1 site was added between the two genes.Source
Codon optimised and synthesised by DNA 2.0| Sequence Annotation | Location | Component / Role(s) |
| rbs Luciferin regenerating enzyme (P.pyralis) double stop codon BamH1 site rbs EPIC luciferase (P. pyralis) double stop codon | 1,17 18,941 942,947 948,953 954,970 971,2620 2621,2626 | ribosome_entry_site feature/rbs feature/protein CDS feature/stop stop_codon non_covalent_binding_site feature/binding feature/rbs ribosome_entry_site sequence_feature feature/misc stop_codon feature/stop |