Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anja Hohmann and Will Handley
Date created: 2010-09-16 11:00:00
Date modified: 2015-05-08 01:12:02
Green Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K325209_sequence (Version 1) |
Description
This is the lux operon from V. fischeri expressed under a pBAD promoter. It emits light when induced by arabinose. luxCDE create a substrate which the luciferase complex of luxA and luxB uses to emit light at a blue wavelength.Notes
Removal of existing lux promoter, and replacement with pBADSource
From pHK555 provided by James Slock ( http://departments.kings.edu/biology/lux/bacterial.html )| Sequence Annotation | Location | Component / Role(s) |
| I0500 AraC PC Pbad rbs Luciferin Regenerating Enzyme (L. cruciata) double stop codon BamH1 site rbs Wild Type Luciferase (L. cruciata) double stop codon | 1,1210 1,1079 1043,1114 1080,1210 1219,1235 1236,2162 2163,2168 2169,2174 2175,2191 2192,3835 3836,3841 | engineered_region feature/BioBrick feature/misc sequence_feature promoter feature/promoter feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein feature/stop stop_codon non_covalent_binding_site feature/binding ribosome_entry_site feature/rbs CDS feature/protein stop_codon feature/stop |