Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hannah Copley and Theo Sanderson
Date created: 2010-09-14 11:00:00
Date modified: 2015-05-08 01:12:02
Red Firefly Luciferase and LRE
L. Cruciata
(E. coli optimised)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K325210_sequence (Version 1) |
Description
This part is contains the coding sequence for a mutant light-emitting enzyme (luciferase) and luciferin regenerating enzyme (LRE) from the Japanese firefly, Luciola cruciata. It will make bacteria emit light at a reddish wavelength when placed under a promoter, provided the media is supplemented with luciferin.The luciferin regenerating enzyme will convert oxyluciferin into CHBT. CHBT is converted to more luciferin in the presence of D-cysteine.
Notes
Codon optimisation, making compatible with BioBrick assembly format.Source
This was codon-optimised for E.coli and syntesised by DNA 2.0.| Sequence Annotation | Location | Component / Role(s) |
| rbs Luciferin regenerating enzyme (L. cruciata) double stop codon BamH1 site rbs Red luciferase (286) (L. cruciata) double stop codon G to A: Ser286Asn | 1,17 18,944 945,950 951,956 957,973 974,2617 2618,2623 1830,1830 | feature/rbs ribosome_entry_site CDS feature/protein feature/stop stop_codon non_covalent_binding_site feature/binding ribosome_entry_site feature/rbs feature/protein CDS stop_codon feature/stop sequence_alteration feature/mutation |