BBa_K325210

BBa_K325210 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K325210
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hannah Copley and Theo Sanderson
Date created: 2010-09-14 11:00:00
Date modified: 2015-05-08 01:12:02

Red Firefly Luciferase and LRE
L. Cruciata
(E. coli optimised)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K325210_sequence (Version 1)

Description

This part is contains the coding sequence for a mutant light-emitting enzyme (luciferase) and luciferin regenerating enzyme (LRE) from the Japanese firefly, Luciola cruciata. It will make bacteria emit light at a reddish wavelength when placed under a promoter, provided the media is supplemented with luciferin.

The luciferin regenerating enzyme will convert oxyluciferin into CHBT. CHBT is converted to more luciferin in the presence of D-cysteine.

Notes

Codon optimisation, making compatible with BioBrick assembly format.

Source

This was codon-optimised for E.coli and syntesised by DNA 2.0.

Sequence Annotation Location Component / Role(s)
rbs
Luciferin regenerating enzyme (L. cruciata)
double stop codon
BamH1 site
rbs
Red luciferase (286) (L. cruciata)
double stop codon
G to A: Ser286Asn
1,17
18,944
945,950
951,956
957,973
974,2617
2618,2623
1830,1830
feature/rbs ribosome_entry_site
CDS feature/protein
feature/stop stop_codon
non_covalent_binding_site feature/binding
ribosome_entry_site feature/rbs
feature/protein CDS
stop_codon feature/stop
sequence_alteration feature/mutation
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K325210/1