BBa_K329006

BBa_K329006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K329006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raphael Pantier

Date created: 2010-10-11 11:00:00

Date modified: 2015-05-08 01:12:03

Weak RBS (B0031) - LacZ alpha fragment (I732006)

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K329006_sequence (Version 1)

Description

LacZ-alpha fragment (BBa_I732006) with a Weak RBS upstream (BBa_B0031). In strains with LacZ-omega (LacZ N-terminal deletion mutants) like DH5alpha, DH10B and Top10, LacZ-alpha restores the beta-galactosidase activity.

We have also designed the same construct with a Strong RBS (BBa_K329005).

Notes

We used BioBrick Standard Assembly.

Source

2010 Distribution kit

Components

Access	Instance	Definition
public	BBa_B0031	BBa_B0031
public	BBa_I732006	lacZ-alpha

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0031	1,14	BBa_B0031
BBa_I732006	21,254	lacZ-alpha

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K329006/1

Member of these Collections

• iGEM Parts Registry