BBa_K329009

BBa_K329009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K329009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raphael Pantier
Date created: 2010-10-11 11:00:00
Date modified: 2015-05-08 01:12:03

attC - double terminator (B0015) - attC - mRFP



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K329009_sequence (Version 1)

Description

This part is composed of a double terminator (BBa_B0015) flanked by attC sites and followed by mRFP (with an RBS). When the IntI integrase is expressed (and if there is a promoter upstream of this part), it can specifically excise the terminator and triggers the expression of mRFP.


Important note : RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix.

Notes

RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix. We used BioBrick Standard Assembly.

Source

BBa_K329008

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K329009/1