Types | DnaRegion
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Roles | engineered_region
Device
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Sequences | BBa_K329009_sequence (Version 1)
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Description
This part is composed of a double terminator (
BBa_B0015) flanked by attC sites and followed by mRFP (with an RBS). When the IntI integrase is expressed (and if there is a promoter upstream of this part), it can specifically excise the terminator and triggers the expression of mRFP.
Important note : RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix.
Notes
RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix. We used BioBrick Standard Assembly.