BBa_K329010

BBa_K329010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K329010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xavier Duportet

Date created: 2010-10-16 11:00:00

Date modified: 2015-05-08 01:12:03

RBS + Int HK022

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K329010_sequence (Version 1)

Description

This integrase comes from the double-stranded HK022 DNA enterobacteriophage of Escherichia coli, member of the lambdoid or lambda-like group of phages.
This protein is necessary for both the integration and the excision of the phage DNA into the host cell.
The integration process only requires Int to be expressed, and recombines the attP site from the phage with the attB attachment site on the bacterial chromosome.

Notes

The original RBS was kept, superposed with the end of the Xis gene.

Source

Parts come from a PCR amplification on a genomic source.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	7,12	ribosome_entry_site, feature/rbs
Int HK022	20,1074	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: P16407
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K329010/1

Member of these Collections

• iGEM Parts Registry