BBa_K329011

BBa_K329011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K329011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xavier Duportet

Date created: 2010-10-16 11:00:00

Date modified: 2015-05-08 01:12:03

RBS + Xis-Int HK022

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K329011_sequence (Version 1)

Description

This part allows the expression of both the Excisionase and the Integrase of the double-stranded HK022 DNA enterobacteriophage of Escherichia coli, member of the lambdoid or lambda-like group of phages.
Expression of these two proteins promotes the excision of the phage DNA by recombining the attR and the attL sites.
After excision, the original attB and attP sites are reformed.

Notes

Kept the original RBS and the structural organisation of these two genes (the beginning of Int overlaps with the end of Xis).

Source

PCR amplification on a genomic source.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS Xis	7,12	ribosome_entry_site, feature/rbs
Xis	20,238	feature/cds, CDS
Int	216,1289	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K329011/1

Member of these Collections

• iGEM Parts Registry