Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: XIE Chensu
Date created: 2010-10-06 11:00:00
Date modified: 2015-05-08 01:12:04
Protein secretor in Lactobacillus Plantarum
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K330001_sequence (Version 1) |
Description
The sequence consists of signal peptide gene lp_0297, Flag-tag sequence, and two enzyme cutting sites (NdeI and HindIII).Bacteria use several pathways for protein export to the membrane, the cell wall or the medium. Many proteins follow the Sec-dependent pathway and are synthesized as precursors with an N-terminal signal peptide that directs the protein to the Sec translocation machinery. Here the signal peptide lp_0297 can help to drive the protein added after it to be secreted out of Lactobacillus Plantarum, and the signal peptide itself will be cleaved off during or shortly after the translocation.The flag-tag added is for the convenience of detection. The two enzyme cutting sites are designed for adding the sequence of proteins to be secreted. We can also choose SpeI and PstI sites to add protein sequence.
Reference:
Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1(Geir Mathiesen*1, Anita Sveen1, May Bente Brurberg2, Lasse Fredriksen1, Lars Axelsson3 and Vincent GH Eijsink1).
Notes
18bp before start codon are chosen as RBS.The original start codon of Signal Peptide lp_0297 is "gtg", which has not been changed to "atg" while making it into our part.
The sequence between NdeI and HindIII is randomly chosen as "ttgata".
Source
The sequence of Signal Peptide lp_0297 came from the genome of Lactobicillus Plantarum WCFS1.The gene sequence of Flag-tag is artificially designed according to its peptide sequence.