BBa_K337003

BBa_K337003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K337003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R

Date created: 2010-10-20 11:00:00

Date modified: 2015-05-08 01:12:06

hsa-miR-122 Binding site pattern (4BS)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K337003_sequence (Version 1)

Description

hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are innert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites.

Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching:
1st: CAAACACCATTGTCACACTC-A
perfect: CAAACACCATTGTCACACTCCA

2nd: CAAACACCATTGTCA-ACTCCA
perfect: CAAACACCATTGTCACACTCCA

Notes

The spacer sequences, present between the miRNA binding sites, should have the lowest compatibility to known miRNAs, to minimize unspecific sequence recognition.

Source

http://mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000421

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Spacer 1st half	1,15	sequence_feature, feature/misc
Spacer 1st half	52,66	sequence_feature, feature/misc
Spacer 1st half	103,117	feature/misc, sequence_feature
Spacer 2nd half	88,102	feature/misc, sequence_feature
Spacer 2nd half	37,51	feature/misc, sequence_feature
Spacer 1st half	155,169	sequence_feature, feature/misc
Spacer 2nd half	140,154	sequence_feature, feature/misc
Spacer 2nd half	192,206	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K337003/1

Member of these Collections

• iGEM Parts Registry