Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R
Date created: 2010-10-20 11:00:00
Date modified: 2015-05-08 01:12:06
hsa-miR-122 Binding site pattern (2BS)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K337004_sequence (Version 1) |
Description
hsa-miR-122 binding site pattern, consisting of 2 perfect matching binding sites. The binding sites are seperated by sequences of 30 base pairs, which are innert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites.Notes
http://mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000421Source
The spacer sequences, present between the miRNA binding sites, should have the lowest compatibility to known miRNAs, to minimize unspecific sequence recognition.| Sequence Annotation | Location | Component / Role(s) |
| Spacer 1st half Spacer 2nd half Spacer 2nd half Spacer 1st half | 1,15 38,52 90,104 53,67 | sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |