BBa_K337005

BBa_K337005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K337005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R

Date created: 2010-10-20 11:00:00

Date modified: 2015-05-08 01:12:06

hsa-miR-122 Binding site pattern (2BS - additional 10bp Spacer)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K337005_sequence (Version 1)

Description

hsa-miR-122 binding site pattern, consisting of 2 perfect matching binding sites. The binding sites are seperated by a sequence of 40 base pairs, which are innert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites.

Notes

The spacer sequences, present between the miRNA binding sites, should have the lowest compatibility to known miRNAs, to minimize unspecific sequence recognition.

Source

http://mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0000421

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Spacer 2nd half	38,52	feature/misc, sequence_feature
addtional 10bp spacer	53,62	sequence_feature, feature/misc
Spacer 2nd half	100,114	sequence_feature, feature/misc
Spacer 1st half	63,77	feature/misc, sequence_feature
Spacer 1st half	1,15	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K337005/1

Member of these Collections

• iGEM Parts Registry