BBa_K337032

BBa_K337032 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K337032

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R

Date created: 2010-10-21 11:00:00

Date modified: 2015-05-08 01:12:06

pSMB_miTuner Plasmid HD1 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv40/CMV/Kozag_hRluc_BGH )

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_K337032_sequence (Version 1)

Description

miTuner plasmid for microRNA binding site measurment and expression tuning.

Notes

easy mesurment of microRNA binding site quality

Source

BGH: pcDNA5/FRT/To

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BGH Terminator	1,255	sequence_feature, feature/misc
synthetic microRNA	261,421	feature/misc, sequence_feature
SV40	427,846	feature/promoter, promoter
RSV	851,1471	feature/promoter, promoter
Luc2	1477,3426	feature/cds, CDS
CMV	3432,4041	promoter, feature/promoter
hRluc	4047,4989	feature/cds, CDS
BGH Terminator	4995,5244	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K337032/1

Member of these Collections

• iGEM Parts Registry