BBa_K337038

BBa_K337038 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K337038

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R

Date created: 2010-10-21 11:00:00

Date modified: 2015-05-08 01:12:06

pSMB_miTuner Plasmid HD4 (BGH(rc)/shRNA10(rc)/RSV(rc)/CMV_TetO2/Luc2_sv40/CMV/Kozag_hRluc_BGH)

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_K337038_sequence (Version 1)

Description

miTuner plasmid HD4 is used for microRNA binding site characterization and expression tuning.

Notes

to be continued

Source

pcDNA5/FRT/TO

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BGH Terminator	1,255	feature/misc, sequence_feature
RSV	427,1048	promoter, feature/promoter
synthetic microRNA	261,421	sequence_feature, feature/misc
CMV_TetO2	1055,1784	feature/promoter, promoter
Luc2_SV40	1790,3739	feature/misc, sequence_feature
CMV	3745,4354	promoter, feature/promoter
hRluc	4360,5302	sequence_feature, feature/misc
BHG Terminator	5308,5557	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K337038/1

Member of these Collections

• iGEM Parts Registry